Mass Spectrometry for Identification- How does it work?

MALDI-TOF (Matrix Assisted Laser Desorption Ionization) is used as a method of rapid microbial identification. LAB 797 utilizes this technology at our facility, allowing us to provide you fast and accurate results.

Samples are prepared for analysis by being coated with an energy-absobent matrix solution. The solution crystalizes upon drying, encasing proteins within it. The matrix is then ionized with a laser beam, the ions are charged, and they are accelerated within the MALDI-TOF device. Ions are seperated based on a mass-to-charge ratio (m/z) and visualized against a known spectra of organisms within a microbial library. Once identified, the system will output an identification for the organism.

The entire process, including preperation time, can deliver a microbial ID, start to finish, in approximately 5-10 minutes.

Other applications for MALDI-TOF include specialty imaging, polymer analysis, quality control, cell research, and proteomics.

Let us work with your facility to ID any of your bacterial or fungal isolates. We have an ID-Only program where you can send us growth from your facility, we work to ID the organisms, and get you results within 72 hours. Contact us to learn more.